Pseudomonas sp., Strain L5B5: A Genomic and Transcriptomic Insight into an Airborne Mine Bacterium

Mines, like other subterranean environments, have ecological conditions which allow the thriving of microorganisms. Prokaryotes and fungi are common inhabitants mines, developing a metabolism suitable for growing in such inhospitable environments. The mine Lousal, Portugal, is an interesting site study microorganisms present their galleries. Aerobiological studies resulted isolation Pseudomonas sp., strain L5B5, closely related to opportunistic fish pathogen P. piscis MC042T, soil bacteria protegens CHA0T, Cab57, Pf-5. Strain L5B5 was able inhibit growth pathogenic Bacillus cereus, Staphylococcus aureus, Acinetobacter baumanii, as well cave Aspergillus versicolor, Penicillium chrysogenum, Cladosporium cladosporioides, Fusarium solani, Ochroconis lascauxensis. In silico analyses based on de novo genome hybrid assembly RNA-Seq, performing seven culture phases prediction detection genetic mechanisms involved secondary metabolites, with presence possible new gene cluster transcribed under tested conditions, feasible virulence factors antimicrobial resistance mechanisms.